normal lung epithelial cell line l132  (National Centre for Cell Science)

Journal: Briefings in Bioinformatics

Article Title: Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis

doi: 10.1093/bib/bbae035

Figure Lengend Snippet: Phenotypes of designated siRNA-transfected cells. ( A ) Effects of siRNAs on F-actin organization in L132 cells in the presence of TGFβ for 24 h. F-actin was visualized by Alexa Fluor™ 488 Phalloidin. The magnification is 40′. ( B ) The migration capacity of siRNA-transfected cells with or without treatment with TGFβ was detected using a wound-healing assay. ( C ) Cell proliferation upon transfection of siRNAs with or without TGFβ treatment was determined by MTT assay ( * P < 0.05, * * P < 0.01, * * * P < 0.001 compared with control cells; # P < 0.05, # # P < 0.01, # # # P < 0.001 compared with cells treated with TGFβ alone).

Article Snippet: A human normal lung epithelial cell line (L132) and HEK 293T cells were supplied by the American Type Culture Collection (Rockville, MD, USA) and cultured in RPMI (Gibco, Gaithersburg, MD, USA) or DMEM supplemented with 10% fetal bovine serum and 1% penicillin–streptomycin at 37°C in a humidified 5% CO 2 incubator.

Techniques: Transfection, Migration, Wound Healing Assay, MTT Assay, Control